SABio: RNA, Antibodies, Peptides and Other Quality Biologics
orders@sabio.com.sg
  • Home
  • Products & Services
    • siRNA & miRNA
    • Tranfection Reagents
    • siRNA Libraries
    • RNA Oligos
    • RNA Gel Markers
    • Antibodies
    • Antibody Production
    • Peptides
    • Human VEGF-C & D
    • How to Order
  • News
  • About Us
  • Contact Us
    • Contact Info
    • Directions
    • Careers at SABio

Custom Antibody Services


We offer quality services and competitive prices for both polyclonal and
monoclonal antibody development.

For enquiries and quotations, please contact us at orders@sabio.com.sg  - we will be happy to assist you  with pricing and tailor the service to your needs.
Request a Quote


Picture

Monoclonal Antibodies

Upfront Fee Services
 
  • Immunization of 4 mice
  • Screening of sera by us after 2nd and 3rd injection for recognition of  antigen
  • Fusion and hybridoma production
  • Screening of hybridomas (dotblot and/or Western or cytochemistry using  your antigen or cells expressing your antigen)
  • Supply of hybridoma supernatants for your tests

Final Fee Services (if approved by cystomer):
 
  • Cloning and recloning of positive hybridoma cells
  • Test of subclass of Ig
  • Production of master stock of hybridoma cells & 1 year free storage
  • Production of 25 ml of each hybridoma supernatant
  • Delivery of hybridoma cells and supernatant
  • Documentation and hybridoma cell description
  •   Standard monoclonal antibody package includes upfront and final  fee services.

Additional  Services

 
  • Antigen peptide synthesis (or you can provide your antigen) 
  • Antibody purification (scale from 2 mg to 100 mg available)
  • Direct labelling of antibodies: Peroxidase, Biotin, etc.
  • Immunochemical analysis of antibody: Immunoprecipitation, Western Blotting,  ELISA
  • Immunohistochemical analysis of antibody: Immunohistochemistry
  • Long term storage of hybridomas

Pricing & Schedule
  
The exact  price depends on the complexity of the project. It is fixed after  the  project is specified in detail. Initial upfront fee payment is due upon confirming the project. Final payment is due after delivery of  successful hybridoma(s). We will communicate with you during the development process to  design the best screening test to get a maximum number of hybridomas. The  schedule can be customized to meet your needs. Approximate  standard schedule is as  follows:

Week 1-12 Immunizations
Week 9-13 Cell fusion
Week 10-14 Screening
Week 12-16 Propagation & testing of hybridomas
Week 13-15 Re-clonin
Week 14-16 Expansion,  supernatant production, delivery
 
Privacy & Rights


Complete  confidentiality is guaranteed and all the intellectual property and  hybridoma usage belongs exclusively to customer. A special agreement can be  
signed upon request.

Polyclonal Antibodies


 Services Available:
 
  • Antigen peptide synthesis or your provided antigen
  • Immunizations - polyclonal sera development
  • Prolongation (extra boost and bleed) 
  • Affinity purification of sera
  • Antibody testing (Western Blotting, Immunoprecipitation,
    Immunocytochemistry, ELISA)

Species available: Rabbit, Mouse, Rat, Guinea Pig, Sheep


Standard Packages

 Production of rabbit polyclonal sera includes:
 
  • 2 - 3 mls of pre-immune sera
  • Preliminary samples (2-5mls/rabbit) after second immunisation (6-7 weeks after first   immunisation).
  • Immune sera (60-80mls/rabbit) (3 months after beginning of immunisation). 
  • Basic testing for level of specific Ig in the sera if you supply us with extra antigen (dot-blot or western blotting).
  • For additional injections of antigen, testing and purification – please enquire.
 
Production of guinea pig polyclonal sera includes:
 
  • 1 ml of pre-immune sera
  • Preliminary samples after second immunisation (6-7 weeks after first immunisation).
  • Immune sera (20-25 mls/guinea pig) - (3 months after beginning of  immunisation).
  • For additional injections of antigen, testing and purification – please  enquire.
 
Production of rat polyclonal sera includes:
 
  • 1 ml of pre-immune sera
  • Preliminary samples after second immunisation (6-7 weeks after first  immunisation).
  • Immune sera (10-15 mls/rat) - (3 months after beginning of  immunisation).
  • For additional injections of antigen, testing and purification – please  enquire.
 
Production of sheep polyclonal sera includes:
 
  • 10ml of pre-immune sera
  • Preliminary samples after second immunisation (6-7 weeks after first  immunisation)
  • Immune sera (300 mls/sheep) - (4 months after beginning of  immunisation).
  • For additional injections of antigen, testing and purification – please enquire.

 
  Amount of Antigen Required per Dose
 
 Rabbit - Peptide Protein  0,20-0,80 - 0,50-1,00 mg
 Guinea pig - Peptide Protein - 0,10-0,20 - 0,15-0,25 mg
 Mouse - Peptide Protein - 0,01-0,03 - 0,03-0,05 mg
 Rat - Peptide Protein - 0,05-0,10 - 0,10-0,15 mg
 Sheep - Peptide Protein 0,30-1,00 - 0,50-2,00 mg
Powered by Create your own unique website with customizable templates.