Custom Antibody ServicesWe offer quality services and competitive prices for both polyclonal and monoclonal antibody development. For enquiries and quotations, please contact us at orders@sabio.com.sg - we will be happy to assist you with pricing and tailor the service to your needs. |
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Monoclonal Antibodies
Upfront Fee Services
Final Fee Services (if approved by cystomer):
Additional Services
Pricing & Schedule
The exact price depends on the complexity of the project. It is fixed after the project is specified in detail. Initial upfront fee payment is due upon confirming the project. Final payment is due after delivery of successful hybridoma(s). We will communicate with you during the development process to design the best screening test to get a maximum number of hybridomas. The schedule can be customized to meet your needs. Approximate standard schedule is as follows:
Week 1-12 Immunizations
Week 9-13 Cell fusion
Week 10-14 Screening
Week 12-16 Propagation & testing of hybridomas
Week 13-15 Re-clonin
Week 14-16 Expansion, supernatant production, delivery
Privacy & Rights
Complete confidentiality is guaranteed and all the intellectual property and hybridoma usage belongs exclusively to customer. A special agreement can be
signed upon request.
- Immunization of 4 mice
- Screening of sera by us after 2nd and 3rd injection for recognition of antigen
- Fusion and hybridoma production
- Screening of hybridomas (dotblot and/or Western or cytochemistry using your antigen or cells expressing your antigen)
- Supply of hybridoma supernatants for your tests
Final Fee Services (if approved by cystomer):
- Cloning and recloning of positive hybridoma cells
- Test of subclass of Ig
- Production of master stock of hybridoma cells & 1 year free storage
- Production of 25 ml of each hybridoma supernatant
- Delivery of hybridoma cells and supernatant
- Documentation and hybridoma cell description
- Standard monoclonal antibody package includes upfront and final fee services.
Additional Services
- Antigen peptide synthesis (or you can provide your antigen)
- Antibody purification (scale from 2 mg to 100 mg available)
- Direct labelling of antibodies: Peroxidase, Biotin, etc.
- Immunochemical analysis of antibody: Immunoprecipitation, Western Blotting, ELISA
- Immunohistochemical analysis of antibody: Immunohistochemistry
- Long term storage of hybridomas
Pricing & Schedule
The exact price depends on the complexity of the project. It is fixed after the project is specified in detail. Initial upfront fee payment is due upon confirming the project. Final payment is due after delivery of successful hybridoma(s). We will communicate with you during the development process to design the best screening test to get a maximum number of hybridomas. The schedule can be customized to meet your needs. Approximate standard schedule is as follows:
Week 1-12 Immunizations
Week 9-13 Cell fusion
Week 10-14 Screening
Week 12-16 Propagation & testing of hybridomas
Week 13-15 Re-clonin
Week 14-16 Expansion, supernatant production, delivery
Privacy & Rights
Complete confidentiality is guaranteed and all the intellectual property and hybridoma usage belongs exclusively to customer. A special agreement can be
signed upon request.
Polyclonal Antibodies
Services Available:
- Antigen peptide synthesis or your provided antigen
- Immunizations - polyclonal sera development
- Prolongation (extra boost and bleed)
- Affinity purification of sera
- Antibody testing (Western Blotting, Immunoprecipitation,
Immunocytochemistry, ELISA)
Species available: Rabbit, Mouse, Rat, Guinea Pig, Sheep
Standard Packages
Production of rabbit polyclonal sera includes:
- 2 - 3 mls of pre-immune sera
- Preliminary samples (2-5mls/rabbit) after second immunisation (6-7 weeks after first immunisation).
- Immune sera (60-80mls/rabbit) (3 months after beginning of immunisation).
- Basic testing for level of specific Ig in the sera if you supply us with extra antigen (dot-blot or western blotting).
- For additional injections of antigen, testing and purification – please enquire.
Production of guinea pig polyclonal sera includes:
- 1 ml of pre-immune sera
- Preliminary samples after second immunisation (6-7 weeks after first immunisation).
- Immune sera (20-25 mls/guinea pig) - (3 months after beginning of immunisation).
- For additional injections of antigen, testing and purification – please enquire.
Production of rat polyclonal sera includes:
- 1 ml of pre-immune sera
- Preliminary samples after second immunisation (6-7 weeks after first immunisation).
- Immune sera (10-15 mls/rat) - (3 months after beginning of immunisation).
- For additional injections of antigen, testing and purification – please enquire.
Production of sheep polyclonal sera includes:
- 10ml of pre-immune sera
- Preliminary samples after second immunisation (6-7 weeks after first immunisation)
- Immune sera (300 mls/sheep) - (4 months after beginning of immunisation).
- For additional injections of antigen, testing and purification – please enquire.
Amount of Antigen Required per Dose
Rabbit - Peptide Protein 0,20-0,80 - 0,50-1,00 mg
Guinea pig - Peptide Protein - 0,10-0,20 - 0,15-0,25 mg
Mouse - Peptide Protein - 0,01-0,03 - 0,03-0,05 mg
Rat - Peptide Protein - 0,05-0,10 - 0,10-0,15 mg
Sheep - Peptide Protein 0,30-1,00 - 0,50-2,00 mg
